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The loss of activity is not really as a consequence of a diminished potential to bind divalent cations For the reason that metal articles from the purified mutants was identical to the wild form enzyme. The catalytic Qualities of such mutants are totally in step with the proposed function of this residue in the chemical system as the first general base for activation of your hydrolytic drinking water molecule and the following protonation on the leaving team amine.
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have to be significantly distinct slot naga26 For the reason that H×H motif from the conclude of β-stand 1 is changed by Q×N and thus it is very probably this enzyme can bind only one divalent cation in the Lively site.
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Incubation from the apo-enzyme with one particular equal of Zn2+ for 30 minutes completely restored catalytic action. The titration of apo-enzyme with varying quantities of ZnCl2 is demonstrated in Determine one. These success reveal that NagA from E. coli
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